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Mouse Heart

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cTnnT+ cells are initially columnar epithelial cells and show apical localization of the tight junction component zona-occluden-1 (ZO-1) ( Figure 1—figure supplement 3B,B’). These 2D image stacks can be readily resliced in any arbitrary imaging plane, and using these 2D image stacks, 3D reconstructions of the specimen can be generated with ease.

cre/+; Rosa26RmT/mG +/- embryo at cc stage and single-section time-lapse video of the same embryo after 10 hr 42 m of ex-vivo culture (representative analysis from three embryos).Until that is, her most beloved friend and adopted guardian, Walter, is accused of a crime that Mouse is sure he didn’t commit. As Mouse's investigation leads her ever closer to their true, deadly nature, can she outwit them without losing everything she holds dear?

We propose that two distinct phases of cardiomyocyte differentiation take place during early heart development. We constructed a developmental atlas of the mouse cardiovascular system using episcopic fluorescence image capture (EFIC), a histological imaging techniques that provides serial 2D histological images that are high resolution and in complete registration. undergoes further morphogenesis to transform into a HT, initially open dorsally and no cardiomyocyte differentiation is detected during this transformation. RERT cre/+; Rosa26Rtdtomato +/- embryo during stages when cc differentiation takes place -from EHF onwards-. D–E) After over 10 hr of ex-vivo culture inside the two-photon chamber, the embryo has grown and the cardiac crescent has transformed into a beating heart tube.Unlike other organs, the heart has to start beating while it is still developing, and until now, it was unclear how the heart manages this difficult task. Mouse knows that Walter would never commit the atrocities of which he has been accused, so she determines to clear his name and free him from gaol. Here, we show the relevance of differentiation timing regulation during heart tube formation and its coordination with morphogenesis at the tissue level.

Cells with GFP levels above the median intensity value of all cells at the end of the recording are represented as green spheres and cells with lower GFP levels are shown as red spheres.In agreement with our previous observations, we found that SHF cells do not differentiate up to the open HT stage, when the dorsal seam of the heart is still open. In ( B, B’) only the double-positive cells located in the linear HT are shown, while the tdtomato+ progenitors, located outside the linear HT are not shown.

When one is imprisoned for murder she has to solve the clues and work out who really commited the crime. We found that the endocardial lumen started to appear while cc cells still remained columnar (compare time points 2 hr 13 m and 3 hr 48 m in Figure 4G’).

Set in a fictional, historical reign of Queen Mary II, there was a swashbuckling, Victorian feel to this book which really created an atmospheric setting! Insets in ( B–D): magnification of single optical sections showing cTnnT localization and cell shape. The right ventricle (RV), the outflow tract and most of the atria derive instead from cardiac progenitors located dorso-medially to the cc in the splanchnic mesoderm, that are progressively recruited at the poles of the HT at subsequent developmental stages ( Cai et al. The initial location of this cell population fated to become cc cardiomyocytes delineates a crescent-shaped domain at EHF stage ( Figure 5H).

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